Clinical characteristics and treatment of eight cases of pemphigus/bullous pemphigoid complicated by herpes simplex virus infection / 中华皮肤科杂志

Objective:To retrospectively analyze clinical characteristics and treatment of pemphigus/bullous pemphigoid (BP) complicated by herpes simplex virus (HSV) infection.Methods:Inpatients with pemphigus/BP complicated by HSV infection were collected from Wuhan No.1 Hospital from 2016 to 2021, and their clinical characteristics, treatment and follow-up results were retrospectively analyzed.Results:Among the 8 patients with pemphigus/BP complicated by HSV infection, there were 2 males and 6 females, and their age was 50.6 ± 8.3 years. Five of them were diagnosed with pemphigus vulgaris (PV), 1 with pemphigus foliaceus (PF), and 2 with BP. Seven were infected with HSV-1, and 1 with HSV-2. All the 8 patients were given systemic glucocorticoids and immunosuppressive agents for the treatment of pemphigus or BP, and were admitted to the hospital due to resistance to the treatment. Seven patients presented with exacerbation or recurrence of primary lesions, and 1 presented with enlarged lesions all over the body. HSV infection-induced lesions were located on the trunk in 4 cases, on the oral mucosa in 4, on the scalp in 3, and on the face in 2; lesions mainly manifested as irregular erosions with blood crusts, and some centrally umbilicated pustules; 7 patients had obvious pain at the lesional sites. During HSV infection, anti-desmoglein 1 antibody levels decreased in all the 6 patients with pemphigus, and anti-desmoglein 3 antibody levels decreased in 4 of the 5 patients with pemphigus vulgaris; anti-BP180 antibody levels decreased in 1 patient with BP, but increased in the other one with BP. After antiviral therapy at adequate doses for adequate durations (7- to 14-day treatment with valacyclovir alone or in combination with ganciclovir), HSV infection was controlled, the autoimmune bullous skin disorder intensity scores decreased compared with those before the antiviral therapy, and pain was significantly relieved in all the patients. No dose adjustment of glucocorticoids or other immunosuppressive agents was made during antiviral therapy in all patients.Conclusion:HSV infection should be considered when patients with pemphigus/BP suffer from recurrence or exacerbation and poorly respond to conventional treatment; for patients with pemphigus/BP complicated by HSV infection, systemic antiviral therapy at adequate doses can be used to control the disease condition without modifying the conventional immunosuppressive regimen.

Pathogenic characteristics of enterovirus infection in Yueqing city from 2010 to 2018 / 中华微生物学和免疫学杂志

Objective To analyze the epidemic characteristics of enterovirus infection in Yueqing city from 2010 to 2018, and to provide reference for the prevention and control of hand, foot and mouth dis-ease (HFMD) caused by enterovirus. Methods Clinical data of HFMD cases caused by enterovirus infec-tion during 2010 to 2018 were derived from the disease monitoring information report management system of Yueqing city. Descriptive epidemiological study was performed to analyze the characteristics of enterovirus infection by season, age, gender and region and the features of pathogens. Results There were53178 pa-tients with HFMD in total, including 75 severe cases and six deaths. Enterovirus infection occurred in every month of the years and peaked from April to July. Most of the cases were scattered children and nursery chil-dren under five years old and the incidence was higher in males than in females. From 2010 to 2018, the characteristics of enterovirus in Yueqing city had changed significantly. Enterovirus 71 (EV71) was the pre-dominant pathogen during 2010 to 2014, but its detection rate had gradually decreased since 2013. In recent years, the incidence of HFMD caused by non-EV71 and non-coxsackievirus A16 (non-CVA16) enterovirus-es significantly increased. Conclusions Enterovirus infection in Yueqing city was featured by significant seasonality and population-specific variation. Etiological detection should be strengthened. Comprehensive prevention and control measures should be taken to prevent the outbreak of HFMD during April to July.

Pathogenic characteristics of enterovirus infection in Yueqing city from 2010 to 2018 / 中华微生物学和免疫学杂志

Objective@#To analyze the epidemic characteristics of enterovirus infection in Yueqing city from 2010 to 2018, and to provide reference for the prevention and control of hand, foot and mouth disease (HFMD) caused by enterovirus.@*Methods@#Clinical data of HFMD cases caused by enterovirus infection during 2010 to 2018 were derived from the disease monitoring information report management system of Yueqing city. Descriptive epidemiological study was performed to analyze the characteristics of enterovirus infection by season, age, gender and region and the features of pathogens.@*Results@#There were 53 178 patients with HFMD in total, including 75 severe cases and six deaths. Enterovirus infection occurred in every month of the years and peaked from April to July. Most of the cases were scattered children and nursery children under five years old and the incidence was higher in males than in females. From 2010 to 2018, the characteristics of enterovirus in Yueqing city had changed significantly. Enterovirus 71 (EV71) was the predominant pathogen during 2010 to 2014, but its detection rate had gradually decreased since 2013. In recent years, the incidence of HFMD caused by non-EV71 and non-coxsackievirus A16 (non-CVA16) enteroviruses significantly increased.@*Conclusions@#Enterovirus infection in Yueqing city was featured by significant seasonality and population-specific variation. Etiological detection should be strengthened. Comprehensive prevention and control measures should be taken to prevent the outbreak of HFMD during April to July.

Pathogenic characteristics of enterovirus infection in Yueqing city from 2010 to 2018 / 中华微生物学和免疫学杂志

Objective@#To analyze the epidemic characteristics of enterovirus infection in Yueqing city from 2010 to 2018, and to provide reference for the prevention and control of hand, foot and mouth disease (HFMD) caused by enterovirus.@*Methods@#Clinical data of HFMD cases caused by enterovirus infection during 2010 to 2018 were derived from the disease monitoring information report management system of Yueqing city. Descriptive epidemiological study was performed to analyze the characteristics of enterovirus infection by season, age, gender and region and the features of pathogens.@*Results@#There were 53 178 patients with HFMD in total, including 75 severe cases and six deaths. Enterovirus infection occurred in every month of the years and peaked from April to July. Most of the cases were scattered children and nursery children under five years old and the incidence was higher in males than in females. From 2010 to 2018, the characteristics of enterovirus in Yueqing city had changed significantly. Enterovirus 71 (EV71) was the predominant pathogen during 2010 to 2014, but its detection rate had gradually decreased since 2013. In recent years, the incidence of HFMD caused by non-EV71 and non-coxsackievirus A16 (non-CVA16) enteroviruses significantly increased.@*Conclusions@#Enterovirus infection in Yueqing city was featured by significant seasonality and population-specific variation. Etiological detection should be strengthened. Comprehensive prevention and control measures should be taken to prevent the outbreak of HFMD during April to July.

Analysis of 298 Cases of New and Severe ADR Induced by TCM for Removing Blood Stasis and Clearing Heat in Our Hospital / 中国药房

OBJECTIVE:To investigate the characteristics and rules of new and severe ADR induced by TCM for removing blood stasis and clearing heat in our hospital,and to provide reference for safe drug use in clinic. METHODS:New and severe ADR induced by TCM for removing blood stasis and clearing heat reported to national ADR monitoring center were collected from our hospital during Jan. 2006-Dec. 2016. Those ADR cases were analyzed retrospectively in respects of gender,age,allergic history,route of administration,drug type and dosage form,occurrence time,organs/systems involved and clinical manifestations, outcome. RESULTS:Among 298 reports,the incidence of ADR in female(70.13%)was higher male(29.87%),especially in patients aged 50-59(21.48%);there were 77 allergic cases(25.84%). Route of administration was mainly oral administration (57.37%),and dosage form was mainly injection(25.50%). Main ADR-inducing drug was Chinese patent medicine(53.53%). ADR mostly occurred over 1 d after medication(48.32%). Systems/organs involved in ADR were mainly lesion of skin and its appendants,systemic damage and gastrointestinal damage. Main clinical manifestations were rash,contact dermatitis,pruritus, infusion reaction,chills,fever,nausea,vomiting,diarrhea,etc.After drug withdrawal or symptomatic treatment,97.99% patients were revered and cured. There was no death case. CONCLUSIONS:TCM for removing blood stasis and clearing heat causes most new and severe ADR.Clinical drug use should be monitored closely to reduce the occurrence of ADR.

A comparison of delayed discount and personality characteristics between male impulsive and premeditated prisoners / 中华行为医学与脑科学杂志

Objective To explore the delayed discount and personality characteristics of impulsive and premeditated male prisoners and their correlation.Methods A total of 120 male prisoners with aggressive behaviors were tested by Self-made General Situation Questionnaire,Impulsive / Premeditated Aggression Scale (IPAS),NEO Five-Factor Inventory (NEO-FFI),Delay Discount Task (DDT).The effective participants were ninety,including 51 in impulsive group and 39 in premeditated group.Analyzed the difference of delayed discount and personality characteristics between the two group.Results (1) In the delayed discount task,the delay discount rat (K) of the impulsive violence group (0.0278 ± 0.0555) was significantly higher than that of the premeditated violence group (0.0042±0.0078).The difference was statistically significant (t=3.004,P =0.004).(2) The scores of agreeableness (35.84 ± 4.08) and conscientiousness (37.04±4.83) in impulsive violence group were lower than that in premeditated violence group(38.46±5.53,42.31±5.96),and the difference was statistically significant (t=-2.587,P=0.011;t =-4.634,P＜0.01).(3) Correlation analysis showed that K value was not significantly correlated with all dimensions of the big five personality(P＞0.05).Conclusion Male impulsive prisoners have faster tendency to delay discounting compared with male premeditated prisoners.Lower agreeableness and conscientiousness are the personality characteristics of male impulsive prisoners.

Curcumin downregulation of MGMT expression to enhance the chemosensitivity of temozolomide on maligant glioblastoma / 国际肿瘤学杂志

Objective To explore the roles of curcumin in regulating high level O6-methylguanine-DNA methyltransferase (MGMT) expression and the chemosensitivity of gliomas cells to temozolomide (TMZ) in malignant gliomas.Methods The expressions of MGMT were detected in C6 and U87 cell lines and primary malignant gliomas cell by qRT-PCR.The cell proliferation was analyzed after added in signal curcumin,signal TMZ,curcumin combined with TMZ in cells by CCK-8 assay.The cell apoptosis rate was detected by flow cytometry.Results Compared with single curcumin (C6:0.64 ±0.03;U87:0.63 ±0.06;primary cell:0.51 ±0.07) and single TMZ (C6:0.53 ±0.06;U87:0.51 ±0.04;primary cell:0.79 ±0.03),curcumin combined with TMZ (C6:0.14 ±0.01;U87:0.12 ±0.03;primary cell:0.29 ±0.02) could significantly reduce the expression of MGMT in C6,U87 cell lines and primary cell line (C6:F=23.675,P=0.006;U87:F=29.021,P=0.001;primary cell:F=25.534,P=0.001).The combination of curcumin and TMZ could significantly inhibit the cells proliferation,decrease the halfinhibition concentration (IC5o) value (C6:F=6.731,P=0.012;U87:F=17.321,P=0.008;primary cell:F=18.857,P =0.007).The apoptosis rate of the cells was significantly increased after the combined action of curcumin and TMZ (C5:F=25.871,P=0.001;U87:F=6.847,P=0.009;primary cell:F=36.641,P=0.000).Conclusion Synergistic effect of curcumin and TMZ can reduce the expression of MGMT and increase the chemosensitivity of TMZ to malignant glioma,and provide new strategy and methods for the treatment of malignant glioma.

Serum-free culture of the MKN-45 gastric cancer cell line and preparation of stem cells / 医学研究生学报

[Abstract ] Objective Difficulties with the preparation of gastric cancer stem cells (CSCs) have been a main obstacle to the studies of gastric cancer .This article addresses the technology of the serum-free medium suspension cultivation of the MKN-45 gastric cancer cell line and screening of stem cells from the cell line based on the biomarkers of gastric CSCs . Methods MKN-45 cells were cultured in serum-free medium for 8 weeks and those in the logarithmic phase cultivated with hoechst 33342 followed by detection of the side cells by flow cytometry .When the side population cells reached 25%, all the cell microspheres were collected , hatched with CD133 and CD44, and subjected to fluorescence-activated cell sorting.The CDl33 +and CD44 +cells were selected as gastric CSCs . Results About 40%of the MKN-45 gastric CSCs were alive , prolif-erated, and formed floating cell balls .Side population cells constitu-ted 3.4% of the MKN-45 cells and 26.9% of the cell balls.The CDl33 +and CD44 +cells made up 11.2% of the MKN-45 cells and 90.3%of the cell balls. Conclusion Cell balls rich in CSCs can be successfully obtained by serum-free medium suspension culti-vation and CSCs can be screened out with hoechst 33342 and surface markers , which may serve as an experimental ground for the stud-ies of gastric CSCs .

The expression of ABCC4/MRP4 and ABCC5/MRP5 gene in the NK/T cell lymphoma and its relationship with clinical efifcacy / 中国癌症杂志

Background and purpose: Natural killer/T cell lymphoma in poor effects, the production of multidrug resistance is one of the reasons to reduce the chemotherapy effect or failure. This study aimed to discuss the multidrug resistance associated protein 4 (ABCC4/MRP4) gene and multidrug resistance associated protein 5 (ABCC5/MRP5) gene expression in NK/T cell lymphoma SNK-6, YTS cell lines and NK/T cell lymphoma tissues, and the relationship between the level of ABCC4/MRP4, ABCC5/MRP5 gene expression and the clinical efifcacy. Methods:Real-time lfuorescence quantitative PCR (Real time-PCR) and immunohistochemical method (IHC) were used to detect the ABCC4/MRP4, ABCC5/MRP5 gene and protein expression. Results:Compared with the normal NK cells, ABCC4/MRP4 and ABCC5/MRP5 gene in SNK-6, YTS cell lines were highly expressed (P<0.05); Compared with rhinitis tissues, the expression of ABCC4/MRP4, ABCC5/MRP5 gene was higher in the NK/T cell lymphoma tissues (P<0.05);The expression level of ABCC4/MRP4 and ABCC5/MRP5 gene was negative correlation with clinical efifcacy (P<0.05). Conclusion: The expression of ABCC4/MRP4 and ABCC5/MRP5 gene affects the of clinical efifcacy of NK/T cell lymphoma.

Neuroprotective mechanisms of histone deacetylase inhibitors in ischemic stroke / 国际脑血管病杂志

Histone deacetylases (HDACs) catalyze the deacetylation of histone and nonhistone proteins. They play important roles in regulating chromatin remodeling and gene transcription,and that aberrant chromatin remodeling and transcriptional dysregulation are associated with many neurodegenerative diseases. Preclinical studies have suggested that histone deacetylases inhibitors (HDACIs) can target diverse pathophysiologies of ischemic stroke,not only reduce neuronal damage and infarct volume,but also promote the neuronal plasticity and functional recovery after ischemia. This article mainly reviews the neuroprotective mechanisms of HDACIs in ischemic stroke.